High Density & Continuous Pulping “KNEADINGPULPER”

Optimal medium use for continuous high density perfusion processes.

For maintenance of high cell density in continuous perfusion processes not only feeding with substrates but also removal of inhibitors and toxic waste products are of special interest. High perfusion rates cause large volumes of product containing medium which have to be processed in product isolation. In order to minimize these volumes concentrated feed solutions of optimized medium are used. ...

Contamination of a high-cell-density continuous bioreactor.

Continuous fermentations were carried out with a recombinant flocculent Saccharomyces cerevisiae strain in an airlift bioreactor. Once operating under steady state at a dilution rate of 0.45 h(-1), the bioreactor was contaminated with Escherichia coli cells. The faster growing E. coli strain was washed out of the bioreactor and the recombinant, slower growing flocculating S. cerevisiae strain r...

Continuous Iterated Density Estimation

Pulping Catalysts in Trees

Several hardwood and softwood trees were analyzed for anthraquinone-type components. Wood samples were reduced to a small size and extracted with an organic solvent; the extracts were then concentrated and analyzed by gas chromatography-mass spectroscopy. Low levels of AQ and anthrone components were detected using a sensitive selected-ion monitoring technique. Ten out of seventeen hardwood sam...

Low Density High Density

Mouse 3T3 cells and their Simian Virus 40-transformed derivatives (3T3SV) were used to assess the relationship of transformation, cell density, and growth control to the cellular distribution of newly synthesized glycosaminoglycan (GAG). Glucosamineand galactosamine-containing G A G were labeled equivalently by [all]glucose regardless of culture type, allowing incorporation into the various G A...